rabbit polyclonal against total egfr sc-03 antibody  (Santa Cruz Biotechnology)

Journal: BioMed Research International

Article Title: Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

doi: 10.1155/2015/657179

Figure Lengend Snippet: Deguelin reduced the expression of phosphorylated IGF1R, p-Akt, and p-ERK and induced apoptosis in SCC-4 cell lines. Subconfluent culture was treated with deguelin at different concentrations for 24 h. Whole-cell extracts were prepared and analyzed by Western blot using antibodies against p-Akt, Akt, p-ERK, and ERK (a); p-EGFR, EGFR, p-IGF1R, and IGF1R (b); and PARP (c-PARP, cleaved PARP; u-PARP, uncleaved PARP; total PARP, sum of cleaved and uncleaved PARP) (c). Total cell extracts from Jurkat cells: serum starved overnight and then treated with Calyculin A was used as positive control (PC) for p-Akt and Akt.

Article Snippet: Mouse monoclonal antibody against phosphorylated-ERK1/2 (p-ERK1/2) and rabbit polyclonal antibody against total EGFR were from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Positive Control

Journal: BioMed Research International

Article Title: Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

doi: 10.1155/2015/657179

Figure Lengend Snippet: Deguelin induced apoptosis by targeting both EGFR-Akt and IGF1R-Akt pathways in HNSCC cell lines. Subconfluent cultures were incubated for 24 h in serum-free medium. After the starvation, cells were treated with 10 μ M deguelin for 1 h. (a) The deguelin-treated SCC-4 cells were incubated for 15 min and 24 h with or without 10 ng/ml of IGF, respectively. (b) The deguelin-treated HSC-4 cells were incubated for 24 h with or without 10 ng/ml of EGF. Whole-cell extracts were analyzed by Western blot using antibodies against p-Akt, Akt, and PARP. (c) HSC-4 cells were treated with deguelin at different concentrations for 24 h in 10% FBS-containing medium. Whole-cell extracts were analyzed by Western blot using antibodies against p-EGFR, EGFR, and PARP (c-PARP, cleaved PARP; u-PARP, uncleaved PARP; total PARP, sum of cleaved and uncleaved PARP).

Article Snippet: Mouse monoclonal antibody against phosphorylated-ERK1/2 (p-ERK1/2) and rabbit polyclonal antibody against total EGFR were from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Incubation, Western Blot